【Author】 ZHEN Qing-qiang;ZHANG Jie;LEI Zhen;LI Meng;Department of Vascular Surgery,Laiwu City People’s Hospital;Department of Medical Supply,the Eighth People’s Hospital of Zhengzhou City;Centralab,Henan Province Hospital of Traditional Chinese Medicine;
【机构】 莱芜市人民医院血管外科; 郑州市第八人民医院药械科; 河南省中医院中心实验室;
【摘要】 目的:探讨在大鼠中瓜蒌皮提取物如何影响同型半胱氨酸诱导的动脉粥样硬化。方法:将60只Wistar大鼠随机分为3组:对照组20只、同型半胱氨酸模型组20只、瓜蒌皮提取物实验组20只,采取1%蛋氨酸喂养诱导动脉粥样硬化模型。检测血清中TC、TG、LDL、Hcy浓度,主动脉组织病理切片观察斑块情况,MTT试剂检测同型半胱氨酸、瓜蒌皮提取物处理后对血管平滑肌细胞增殖影响,RT-PCR检测同型半胱氨酸、瓜蒌皮提取物处理后血管平滑肌细胞内c-myc、c-fos基因的表达情况。结果:同型半胱氨酸模型组血清中TC、TG、LDL、Hcy浓度显著高于实验组和对照组(P<0.05),实验组和对照组差异无统计学意义(P>0.05)。主动脉组织切片显示实验组动脉粥样硬化斑块得到显著改善。MTT细胞增殖检测发现,高同型半胱氨酸处理血管平滑肌细胞后,细胞增殖速度显著高于对照组和实验组(P<0.05),而实验组和对照组间差异无统计学意义(P>0.05)。高同型半胱氨酸处理血管平滑细胞后,细胞内c-myc、c-fos基因表达显著高于对照组和实验组(P<0.05),而实验组和对照组间差异无统... 更多
【Abstract】 Objective: To investigated the effect of extractive of pericarpium trichosanthis( EPT) in atherosclerosis induced by homocysteine in rats. Methods: 60 Wistar rats were randomly divided into three groups: control group,homocysteine model group,EPT experimental group,and 20 rats in each group. And 1% methionine diet was fed to rats to induce atherosclerosis model. The serum TC,TG,LDL and Hcy concentration were detected by instrument. The change of aortic plaque was observed from aorta tissue patho... 更多
【关键词】 瓜蒌皮提取物; 同型半胱氨酸; 动脉粥样硬化; c-myc; c-fos; 大鼠;
【Key words】 extractiveofpericarpiumtrichosanthis; homocysteine; atherosclerosis; c-myc; c-fos; rats;
【分类号】R341.1; R285.539.6; R543.5;
【中文主题词】高半胱氨酸*;瓜蒌皮*;动脉粥样硬化*;基因, fos;对照组;大鼠, Wistar;主动脉;模型, 心血管;细胞增殖;统计学
【英文主题词】Homocysteine*;Pericarpium Trichosanthis*;Atherosclerosis*;Genes, fos;Control Groups;Rats, Wistar;Aorta;Models, Cardiovascular;Cell Proliferation;Statistics
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