半夏泻心汤的不传之秘？偷偷告诉你

药

量

“中药的不传之秘在于药量”

方歌好背，药量难学，这是中医人永远的痛，药量始终被中医人称为“不传之秘”，传方不传药，常常使用同样的方剂，疗效却差异巨大。

比如，《伤寒杂病论》中的半夏泻心汤

半夏泻心汤

《伤寒论》149条：“伤寒五六日，呕而发热者，柴胡汤证具，而以他药下之，柴胡汤证仍在者，复与柴胡汤。此虽已下之，不为逆，必蒸蒸而振，却发热汗出而解，若心下满而鞭痛者，此为结胸也，大陷胸汤主之；但满而不痛者，此为痞，柴胡不中与之，宜半夏泻心汤。”

《金匮要略》云：“呕而肠鸣，心下痞者，半夏泻心汤主之。”

半夏（半升，洗） 黄芩 干姜 人参 甘草（炙）各三两 黄连（一两）大枣（十二枚，擘）

以水一斗,煮取六升,去滓,再煎取三升。温服一升,日三服。

半夏和黄连，这一对儿奇妙的组合

为什么以这样的比例出现呢？

让我们一起探寻

医圣张仲景的智慧……

Study of dual-directional regulatory effect of Banxia (Pinellia ternata) and Huanglian (Coptis chinensis) drug pair on gastrointestinal movement of mice

Yue Ji^1#, Jun-Chen Li^1#, Jing-Yan Meng^1*, Xue-Rou Yan¹, Jian-Liang Li¹, Qing-Yun Zhao¹, Kang Yang¹,Chun-Liu Liu¹

¹Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Highlights

Banxia (Pinellia Ternate) and Huanglian (Coptis chinensis) drug pair has dual-directional regulatory effects on gastrointestinal movement in mice with atropine or neostigmine induced functional dyspepsia by modulating GAS, VIP, and SS levels in the intestine.

Editor’s Summary

Banxia (Pinellia ternata) and Huanglian (Coptis chinensis) drug pair was derived from Banxiaxiexin soup, which was first recorded in the Shanghanlun composed by Zhang Zhongjing in the third century (Eastern Han Dynasty of China).

摘要

目的：观察半夏-黄连药对对功能性消化不良小鼠胃肠运动的调节作用。

方法：采用三种不同配比的半夏-黄连药液(1:1, 3:1, 4:1)予昆明种雄性小鼠灌胃。10天后用阿托品腹腔注射法复制胃肠运动抑制模型,新斯的明腹腔注射法复制胃肠运动亢进模型，并且检测其小肠推进率。此外，使用与第一个实验相同的建模方法，对半夏-黄连3:1配比组设立高、中、低三种剂量浓度(4.68 g / L，2.34 g / L和1.17 g / L)。运用酶联免疫吸附试验检测小肠组织内胃肠激素如胃泌素、血管活性肠肽和生长抑素的含量。

结果：在新斯的明诱导的胃肠运动亢进模型中，3 种不同配比的半夏-黄连药液(1:1, 3:1, 4:1)均能显著降低小鼠的小肠推进率(P = 0.003, P = 0.012, P = 0.021)；在阿托品诱导的胃肠运动抑制模型组中，仅 3：1 配比的半夏-黄连药液能显著增加模型小鼠的小肠推进率。在新斯的明诱导的胃肠运动亢进模型中，半夏-黄连药液低剂量组、中剂量组和高剂量组的胃泌素水平均低于模型组(P = 0.001, P = 0.004, P = 0.003)。半夏-黄连药液中剂量组和高剂量组的血管活性肠肽水平均高于模型组(P = 0.004, P = 0.002)。半夏-黄连药液中剂量组的生长抑素水平高于模型组(P = 0.002)；在阿托品诱导的胃肠运动抑制模型中，半夏-黄连药液中剂量组和高剂量组的胃泌素水平均高于模型组(P = 0.007, P = 0.021)。半夏-黄连药液低剂量组，中剂量组和高剂量组的血管活性肠肽水平均高于模型组(P = 0.001, P = 0.001, P = 0.001)。半夏-黄连药液中剂量组的生长抑素水平低于模型组(P = 0.001 和 P = 0.006)。

结论：半夏-黄连可以通过影响小肠组织内的胃泌素、血管活性肠肽和生长抑素的水平来双向调节由阿托品及新斯诱导的小鼠功能性消化不良。

关键词：功能性消化不良；中药；半夏；黄连

Abstract

Objective: To evaluate the regulatory effects of Banxia (Pinellia ternata, P) and Huanglian (Coptis chinensis, C) drug pair, derived from Banxiaxiexin soup, on the gastrointestinal movement of mice with functional dyspepsia. Methods: Mice were treated with different proportions of the P and C drug pair (1:1, 3:1, and 4:1) for 10 days, and subsequently injected with atropine (ATR) or neostigmine (NEO). The effects of the different proportions of P and C were evaluated based on the alvine advance rate. In addition, we used the same modeling method used in the first experiment and administered P: C at ratio of 3:1 and at different doses respectively (4.68 g/L, 2.34 g/L, and 1.17 g/L), and tested levels of the gastrointestinal hormones, gastrin (GAS), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) in the small intestinal tissue using an enzyme-linked immunosorbent assay. Results: In the groups of NEO-induced mice, P:C at ratios of 1:1, 3:1, and 4:1 significantly reduced the alvine advance rate compared with the NEO model group (P = 0.003, P = 0.012 and P = 0.021, respectively). In the groups of ATR-induced mice, only P:1 at ratio of 3:1 significantly increased the alvine advance rate compared with the ATR model group (P = 0.007). After exposure to P: C at ratio of 3:1 and at different dose, the GAS level was lower in the low-, medium-, and high-dose NEO groups than that in the NEO model group (P = 0.001, P = 0.004, and P = 0.003, respectively). The VIP levels were higher in the medium-and high-dose NEO groups than that in the NEO model group (P = 0.004 and P = 0.002, respectively). In addition, the SS level increased in the NEO medium-dose group compared with that in the NEO model group (P = 0.002). The GAS level was higher in the ATR medium- and high-dose groups than in the ATR model group (P = 0.007 and P = 0.021, respectively). The VIP level was lower in the ATR low-, medium-, and high-dose than that in the ATR model group (P = 0.001, P = 0.001, and P = 0.001, respectively). Furthermore, the SS level was lower in the ATR medium- and high-dose groups than that in the ATR model group (P = 0.001 and P = 0.006). Conclusion: The PC drug pair bidirectionally adjusted the NEO- and ATR-induced functional dyspepsia in mice by modulating GAS, VIP, and SS levels in the intestine. 

Cite this article:

Ji Yue, Li Jun-Chen, Meng Jing-Yan, Yan Xue-Rou, Li Jian-Liang, Zhao Qing-Yun, Yang Kang, Liu Chun-Liu. Study of dual-directional regulatory effect of Banxia (Pinellia ternata) and Huanglian (Coptis chinensis) drug pair on gastrointestinal movement of mice. Traditional Medicine Research, 2018, 3(3): 148-156.