miRNA160/ARF抑制棉花种皮生长，导致种子变小

靶向生长素反应因子（ARF）的microRNA 160（miR160）在植物发育中起着许多作用。我们调查了miR160 / ARF在调节棉花种子大小中的作用。抑制miR160活性，特别是在种皮中，由于母体外皮的生长减慢，导致种子更小，纤维产量更少。

扫描电子显微镜和组织学显示，在miR160抑制的细胞系中，被膜中细胞的扩增受到抑制。

 miR160靶向了四个GhARF基因，并在miR160抑制的系中上调了基因，表明棉花中存在miR160 / ARF。

在miR160抑制的系中下调了与种子发育降低相关的五个基因（Ghir_A05G003740，Scaffold1878G000010，Ghir_D09G024980，Ghir_A11G010730和Ghir_A05G041590）。

我们的结果表明，miR160 / ARF通过直接或间接调节种子发育相关的基因来控制母体珠被的生长以影响种子大小。

microRNA 160 (miR160), targeting auxin response factors (ARFs), plays many roles in plant development. We investigated the role of the miR160/ARF axis in regulation of cotton seed size. Suppressing miR160 activity, specifically in the seed coat, led to smaller seeds and less fiber production owing to attenuated growth of the maternal integument. Scanning electron microscopy and histology showed that expansion of cells in the integument was retarded in miR160-suppressed lines. Four GhARF genes were targeted by miR160 and were upregulated in miR160-suppressed lines, indicating that a miR160/ARF axis is present in cotton. Five genes (Ghir_A05G003740, Scaffold1878G000010, Ghir_D09G024980, Ghir_A11G010730, and Ghir_A05G041590), associated with reduced seed development were downregulated in miR160-suppressed lines. Our results suggest that the miR160/ARF axis controls maternal integument growth to influence seed size by directly or indirectly regulating seed development-associated genes.

通讯作者：Lili Tu

华中农业大学农作物遗传改良国家重点实验室，武汉430070，湖北

链接：

https://doi.org/10.1016/j.cj.2019.12.004

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