作物生产力取决于分生组织的活动，分生组织产生优化的植物结构，包括玉米穗的结构。全面了解发育需要了解细胞类型和发育域的多样性以及指定它们所需的基因网络。到目前为止，这些基因主要是通过形态学和经典遗传学的见解来鉴定的，而传统遗传学受到遗传冗余和多效性的限制。
本项目，我们研究了12525个来自玉米穗发育的单个细胞的转录谱。由此产生的发育图谱提供了花序的单细胞RNA测序（scRNA-seq）图谱。我们通过mRNA原位杂交和流式荧光活化细胞分选（FACS）RNA-seq验证了我们的研究结果，并通过预测遗传冗余、整合转录网络和识别与作物产量性状相关的候选基因来展示这些数据如何促进遗传研究。
图 玉米穗原生质体分离构建单细胞转录图谱
Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required to specify them. Until now, these were identified primarily by morphology and insights from classical genetics, which are limited by genetic redundancy and pleiotropy. Here, we investigated the transcriptional profiles of 12,525 single cells from developing maize ears. The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence. We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq, and we show how these data may facilitate genetic studies by predicting genetic redundancy, integrating transcriptional networks, and identifying candidate genes associated with crop yield traits.
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