南京大学：IBD患者的口腔菌群失调 | 热心肠日报

Genomics

[IF:6.205]

High-throughput sequencing provides insights into oral microbiota dysbiosis in association with inflammatory bowel disease

高通量测序揭示IBD与口腔菌群失调存在关联性

10.1016/j.ygeno.2020.09.063

10-01, Article

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Although the prevalence of inflammatory bowel disease (IBD) has been increasing worldwide, the etiology remains elusive. Investigating oral microbiota dysbiosis is essential to understanding IBD pathogenesis. Our study evaluated variations in salivary microbiota and identified potential associations with IBD. The saliva microbiota of 22 IBD patients and 8 healthy controls (HCs) was determined using 16S ribosomal RNA (rRNA) gene sequencing and analyzed using QIIME2. A distinct saliva microbiota dysbiosis in IBD, characterized by alterations in microbiota biodiversity and composition, was identified. Saccharibacteria (TM7), Absconditabacteria (SR1), Leptotrichia, Prevotella, Bulleidia, and Atopobium, some of which are oral biofilm-forming bacteria, were significantly increased. Moreover, levels of inflammatory cytokines associated with IBD were elevated and positively correlated with TM7 and SR1. Functional variations include down-regulation of genetic information processing, while up-regulation of carbohydrate metabolism and protein processing in the endoplasmic reticulum in IBD. Our data implicate salivary microbiota dysbiosis involving in IBD pathogenesis.

First Authors:
Ying Qi

Correspondence Authors:
Fangyu Wang

All Authors:
Ying Qi,Sheng-qi Zang,Juan Wei,Hong-chuan Yu,Zhao Yang,Huimin Wu,Ying Kang,Hui Tao,Miao-fang Yang,Lei Jin,Ke Zen,Fangyu Wang