国内团队：灭活益生菌的生物膜增强骨植入物的骨整合 | 热心肠日报

工程化改造的益生菌生物膜可通过免疫调节和抗感染加强骨整合

10.1126/sciadv.aba5723

11-13, Article

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Preventing multidrug-resistant bacteria–related infection and simultaneously improving osseointegration are in great demand for orthopedic implants. However, current strategies are still limited to a combination of non–U.S. Food and Drug Administration–approved antibacterial and osteogenic agents. Here, we develop a food-grade probiotic–modified implant to prevent methicillin-resistant Staphylococcus aureus (MRSA) infection and accelerate bone integration. Lactobacillus casei is cultured on the surface of alkali heat–treated titanium (Ti) substrates and inactivated by ultraviolet irradiation to avoid sepsis induced by viable bacteria. This inactivated L. casei biofilm shows excellent 99.98% antibacterial effectiveness against MRSA due to the production of lactic acid and bacteriocin. In addition, the polysaccharides in the L. casei biofilm stimulate macrophages to secrete abundant osteogenic cytokines such as oncostatin M and improve osseointegration of the Ti implant. Inactivated probiotics modification can be a promising strategy to endow implants with both excellent self-antibacterial activity and osteointegration ability.

First Authors:
Lei Tan,Jieni Fu,Fan Feng

Correspondence Authors:
Xiangmei Liu,Shuilin Wu

All Authors:
Lei Tan,Jieni Fu,Fan Feng,Xiangmei Liu,Zhenduo Cui,Bo Li,Yong Han,Yufeng Zheng,Kelvin Wai Kwok Yeung,Zhaoyang Li,Shengli Zhu,Yanqin Liang,Xiaobo Feng,Xianbao Wang,Shuilin Wu