Daytime-restricted feeding enhances running endurance without prior exercise in mice
作者：Xin, Haoran, Huang, Rongfeng, Zhou, Meiyu, Chen, Jianghui, Zhang, Jianxin, Zhou, Tingting, Ji, Shushen, Liu, Xiao, Tian, He, Lam, Sin Man, Bao, Xinyu, Li, Lihua, Tong, Shifei, Deng, Fang, Shui, Guanghou, Zhang, Zhihui, Wong, Catherine C. L., Li, Min-Dian
Nature Metabolism：2023/06/26
摘要：
Physical endurance and energy conservation are essential for survival in the wild. However, it remains unknown whether and how meal timing regulates physical endurance and muscle diurnal rhythms. Here, we show that day/sleep time-restricted feeding (DRF) enhances running endurance by 100% throughout the circadian cycle in both male and female mice, compared to either ad libitum feeding or night/wake time-restricted feeding. Ablation of the circadian clock in the whole body or the muscle abolished the exercise regulatory effect of DRF. Multi-omics analysis revealed that DRF robustly entrains diurnal rhythms of a mitochondrial oxidative metabolism-centric network, compared to night/wake time-restricted feeding. Remarkably, muscle-specific knockdown of the myocyte lipid droplet protein perilipin-5 completely mimics DRF in enhancing endurance, enhancing oxidative bioenergetics and outputting rhythmicity to circulating energy substrates, including acylcarnitine. Together, our work identifies a potent dietary regimen to enhance running endurance without prior exercise, as well as providing a multi-omics atlas of muscle circadian biology regulated by meal timing.
身体耐力和能量保存对野外生存至关重要。然而，目前尚不清楚用餐时间是否以及如何调节身体耐力和肌肉昼夜节律。在这里，我们发现，与随意喂食或夜间/清醒时间限制喂食相比，白天/睡眠时间限制喂食（DRF）在雄性和雌性小鼠的整个昼夜节律周期中都能提高100%的跑步耐力。全身或肌肉中昼夜节律时钟的消融消除了DRF的运动调节作用。多组学分析显示，与夜间/清醒时间限制的喂养相比，DRF有力地携带了线粒体氧化代谢中心网络的昼夜节律。值得注意的是，肌肉特异性敲低肌细胞脂滴蛋白周脂素-5在增强耐力、增强氧化生物能量学和向循环能量底物（包括酰基肉碱）输出节律性方面完全模仿DRF。总之，我们的工作确定了一种有效的饮食方案，可以在没有事先锻炼的情况下增强跑步耐力，并提供了一个受用餐时间调节的肌肉昼夜节律生物学的多组学图谱。
