28个免疫基因集把CRC区分成为有生存差异的两类

上午我在生信技能树的推文留下了一个悬念：CIBERSORT根据LM22来分类 就是提到了不仅仅是LM22这样的基因集，还有另外两个也很出名，现在我们介绍一下发表在免疫杂志October 2013,的文章Spatiotemporal Dynamics of Intratumoral Immune Cells Reveal the Immune Landscape in Human Cancer 根据公共数据库的 28 cell types ，定下来了 Five hundred seventy-seven cell-type-specific genes (681 Affymetrix probes) 。

然后根据这 28 cell types 的基因可以把CRC分成两类，如下：

We then performed microarray expression experiments and analyzed the 681 probes in tumors from 105 CRC patients and five histologically normal colon mucosa. Two clusters of patients were found, and 372 probes were significantly differentially expressed between the two groups (Figure 1B).

两组病人高表达的基因分别是：

• An overrepresentation of highly expressed genes specific for T cell populations and subpopulations of Th1, Tγδ, cytotoxic T cells as well as for macrophages and mast cells was significantly associated with the patient cluster 1 (all p values < 0.05).

• In comparison, patient cluster 2 showed an overrepresentation of highly expressed genes specific for eosinophil, Tcm, Th2, Th17, Treg, and NK cell subpopulations (all p values < 0.05).

他们两组病人有统计学显著的生存差异：

而且不仅仅是表达芯片数据，后来还引入了qPCR数据和TMA数据。

这577个基因，在文章附件表格里面有, 现在新的任务来了，我介绍了3个免疫细胞基因集，你是不是应该发挥主观能动性去检查一下它们之间的overlap情况？是不是应该是把不同基因集在不同数据集里面测试验证一下?