ggplot2学习笔记之图形排列

转自 生信宝典​ 作者：严涛 浙江大学作物遗传育种在读研究生（生物信息学方向）伪码农，R语言爱好者，爱开源 简介 R语言基本绘图函数中可以利用par()以及layout()来进行图形排列，但是这两个函数对于ggplot图则不太适用，本文主要讲解如何对多ggplot图形多页面进行排列。主要讲解如何利用包gridExtra、cowplot以及ggpubr中的函数进行图形排列。 绘制图形#load packages library(gridExtra) library(cowplot) library(ggpubr) #dataset ToothGrowth and mtcars mtcars$name <- rownames(mtcars) mtcars$cyl <- as.factor(mtcars$cyl) head(mtcars[, c("name", "wt","mpg", "cyl")])

#First let's create some plots #Box plot(bxp) bxp <- ggboxplot(ToothGrowth, x="dose", y="len", color = "dose", palette = "jco") #Dot plot(dp) dp <- ggdotplot(ToothGrowth, x="dose", y="len", color = "dose", palette = "jco", binwidth = 1) #An ordered Bar plot(bp) bp <- ggbarplot(mtcars, x="name", y="mpg", fill="cyl", #change fill color by cyl color="white", #Set bar border colors to white palette = "jco", #jco jourbal color palette sort.val = "asc", #Sort the value in ascending order sort.by.groups = TRUE, #Sort inside each group x.text.angle=90 #Rotate vertically x axis texts ) bp+font("x.text", size = 8)

#Scatter plots(sp) sp <- ggscatter(mtcars, x="wt", y="mpg", add = "reg.line", #Add regression line conf.int = TRUE, #Add confidence interval color = "cyl", palette = "jco",#Color by group cyl shape = "cyl" #Change point shape by groups cyl )+ stat_cor(aes(color=cyl), label.x = 3) #Add correlation coefficientsp

图形排列 多幅图形排列于一面 ggpubr::ggarrange()ggarrange(bxp, dp, bp+rremove("x.text"), labels = c("A", "B", "C"), ncol = 2, nrow = 2)

cowplot::plot.grid()plot_grid(bxp, dp, bp+rremove("x.text"), labels = c("A", "B", "C"), ncol = 2, nrow = 2)

gridExtra::grid.arrange()grid.arrange(bxp, dp, bp+rremove("x.text"), ncol=2, nrow=2)

排列图形注释 ggpubr::annotate_figure()figure <- ggarrange(sp, bp+font("x.text", size = 10), ncol = 1, nrow = 2) annotate_figure(figure, top=text_grob("Visualizing mpg", color = "red", face = "bold", size=14), bottom = text_grob("Data source:\n mtcars data set", color = "blue", hjust = 1, x=1, face = "italic", size=10), left = text_grob("Figure arranged using ggpubr", color = "green", rot = 90), right = "I'm done, thanks :-)!", fig.lab = "Figure 1", fig.lab.face = "bold")

绘图面板对齐 绘制生存曲线library(survival) head(colon[, c(1:4)]) #Fit survival curves fit <- survfit(Surv(time, status)~adhere, data = colon) library(survminer) ggsurv <- ggsurvplot(fit, data = colon, palette = "jco", #jco palette pval = TRUE, pval.coord=c(500, 0.4), #Add p-value risk.table = TRUE #Add risk table) names(ggsurv) `## [1] "plot" "table" "data.survplot" "data.survtable" ggsurv是一个包含两部分的list plot：生存曲线 table：风险表可以用ggarrange()进行排列这两者ggarrange(ggsurv$plot, ggsurv$table, heights = c(2, 0.7), ncol = 1, nrow = 2) 上图中的坐标轴没有对齐，可以通过参数align来设置ggarrange(ggsurv$plot, ggsurv$table, heights = c(2, 0.7), ncol = 1, nrow = 2, align = "v")

改变排列图的行列 设置面板为两行两列，其中sp占据第一行的两列，bxp以及dp置于第二行的两列ggarrange(sp, #First row with scatter plot(sp) ggarrange(bxp, dp, ncol = 2, labels = c("B","C")),#Second row with box and dot plot nrow = 2, labels = "A" #Labels of the scatter plot)

R包cowplot cowplot::ggdraw()可以将图形置于特定位置, ggdraw()首先会初始化一个绘图面板， 接下来draw_plot()则是将图形绘制于初始化的绘图面板中，通过参数设置可以将图形置于特定位置。draw_plot(plot, x=0, y=0, width=1, height=1) 其中： plot:将要放置的图形 x,y:控制图形位置 width,height:图形的宽度和高度 draw_plot_label():为图形添加标签draw_plot_label(label, x=0, y=1, size=16, ...) 其中： label:标签 x,y:控制标签位置 size:标签字体大小 下面通过一个例子来讲解如何将多个图形放置在特定的位置。ggdraw()+ draw_plot(bxp, x=0, y=0.5, width=0.5, height = 0.5)+ draw_plot(dp, x=0.5, y=0.5, width = 0.5, height = 0.5)+ draw_plot(bp, x=0, y=0, width = 1.5, height = 0.5)+ draw_plot_label(label = c("A", "B", "C"), size = 15, x=c(0, 0.5, 0), y=c(1, 1, 0.5))

R包gridExtra gridExtra::arrangeGrop()改变行列分布 下面将sp置于第一行并横跨两列，而bxp和dp分别分布于第二行两列grid.arrange(sp, #First row with one plot spaning over 2 columns arrangeGrob(bxp, dp, ncol = 2), #Second row with 2plots in 2 different columns nrow=2) #number of rows

也可以通过函数grid.arrange中的layout_matrix来设置复杂的图形布局grid.arrange(bp, #bar plot spaning two columns bxp, sp, #box plot amd scatter plot ncol=2, nrow=2, layout_matrix=rbind(c(1, 1), c(2, 3)))

要相对grid.arrange()以及arrangeGrob()的输出进行注释，首先要利用as_ggplot()将其转化为ggplot图形，进而利用函数draw_plot_label()对其进行注释。gt <- arrangeGrob(bp, bxp, sp, layout_matrix = rbind(c(1,1),c(2, 3))) p <- as_ggplot(gt)+ draw_plot_label(label = c("A", "B", "C"), size = 15, x=c(0, 0, 0.5), y=c(1, 0.5, 0.5)) p

R包grid R包grid中的grid.layout()可以设置复杂的图形布局，viewport()可以定义一个区域用来安置图形排列，print()则用来将图形置于特定区域。 总结起来步骤如下： 创建图形p1,p2,p3,… grid.newpage()创建一个画布 创建图形布局，几行几列 定义布局的矩形区域 print：将图形置于特定区域library(grid) #Move to a new page grid.newpage() #Create layout:nrow=3, ncol=2 pushViewport(viewport(layout = grid.layout(nrow=3, ncol=2))) #A helper function to define a region on the layout define_region <- function(row, col){ viewport(layout.pos.row = row, layout.pos.col = col)} #Arrange the plots print(sp, vp=define_region(row=1, col=1:2)) #Span over two columns print(bxp, vp=define_region(row=2, col=1)) print(dp, vp=define_region(row=2, col=2)) print(bp+rremove("x.text"), vp=define_region(row=3, col=1:2)) 设置共同图例 ggpubr::ggarrange()可以为组合图形添加共同图例 common.legeng=TRUE:在图形旁边添加图例 legend：指定legend的位置，主要选项有：top、bottom、left、right。ggarrange(bxp, dp, labels = c("A", "B"), common.legend = TRUE, legend = "bottom")

含有边际密度图的散点图sp <- ggscatter(iris, x="Sepal.Length", y="Sepal.Width", color="Species", palette = "jco", size=3, alpha=0.6)+border() #Marginal density plot of x(top panel) and y(right panel) xplot <- ggdensity(iris, "Sepal.Length", fill="Species",palette = "jco") yplot <- ggdensity(iris, "Sepal.Width", fill="Species", palette = "jco")+rotate() #Clean the plots xplot <- xplot+clean_theme() yplot <- yplot+clean_theme() #Arrange the plots ggarrange(xplot, NULL, sp, yplot, ncol = 2, nrow = 2, align = "hv", widths = c(2, 1), heights = c(1, 2), common.legend = TRUE)

ggplot图、文本、表格组合density.p <- ggdensity(iris, x="Sepal.Length", fill="Species", palette = "jco") #Compute the summary table of Sepal.Length stable <- desc_statby(iris, measure.var = "Sepal.Length", grps = "Species") stable <- stable[, c("Species", "length", "mean", "sd")] #Summary table plot, medium and theme stable.p <- ggtexttable(stable, rows = NULL, theme = ttheme("mOrange")) text <- paste("iris data set gives the measurements in cm", "of the variables sepal length and width", "and petal length and width, respectively,", "for 50 flowers from each of 3 species of iris.", "The species are Iris setosa, versicolor, and virginica.", sep = " ") text.p <- ggparagraph(text = text, face = "italic", size = 11, color = "black") #Arrange the plots on the same page ggarrange(density.p, stable.p, text.p, ncol = 1, nrow = 3, heights = c(1, 0.5, 0.3))

ggplot图形中嵌入图形元素 ggplot2::annotation_custom()可以添加各种图形元素到ggplot图中annotation_custom(grob, xmin, xmax, ymin, ymax) 其中： grob:要添加的图形元素 xmin, xmax: x轴方向位置（水平方向） ymin, ymax: y轴方向位置（竖直方向） ggplot图形中添加tabledensity.p+annotation_custom(ggplotGrob(stable.p), xmin = 5.5, xmax = 8, ymin = 0.7)

ggplot图形中添加box plotsp <- ggscatter(iris, x="Sepal.Length", y="Sepal.Width", color = "Species", palette = "jco", size = 3, alpha=0.6) xbp <- ggboxplot(iris$Sepal.Length, width = 0.3, fill = "lightgray")+ rotate()+theme_transparent() ybp <- ggboxplot(iris$Sepal.Width, width = 0.3, fill="lightgray")+theme_transparent() # Create the external graphical objects # called a "grop" in Grid terminology xbp_grob <- ggplotGrob(xbp) ybp_grob <- ggplotGrob(ybp) #place box plots inside the scatter plot xmin <- min(iris$Sepal.Length) xmax <- max(iris$Sepal.Length) ymin <- min(iris$Sepal.Width) ymax <- max(iris$Sepal.Width) yoffset <- (1/15)*ymax xoffset <- (1/15)*xmax # Insert xbp_grob inside the scatter plots p+annotation_custom(grob = xbp_grob, xmin = xmin, xmax = xmax, ymin = ymin-yoffset, ymax = ymin+yoffset)+ # Insert ybp_grob inside the scatter plot annotation_custom(grob = ybp_grob, xmin = xmin-xoffset, xmax=xmin+xoffset, ymin=ymin, ymax=ymax)

ggplot图形添加背景#import the imageimg.file <- system.file(file.path("images", "background-image.png"), package = "ggpubr") img <- png::readPNG(img.file) 利用ggpubr::background_image()为ggplot图形添加背景图library(ggplot2) library(ggpubr) ggplot(iris, aes(Species,Sepal.Length))+ background_image(img)+ geom_boxplot(aes(fill=Species), color="white")+ fill_palette("jco")

修改透明度ggplot(iris, aes(Species,Sepal.Length))+ background_image(img)+geom_boxplot(aes(fill=Species), color="white", alpha=0.5)+ fill_palette("jco")

多页排列 日常工作中我们有时要绘制许多图，假如我们有16幅图，每页排列4张的话就需要4页才能排完，而ggpubr::ggarrange()可以通过制定行列数自动在多页之间进行图形排列multi.page <-ggarrange(bxp, dp, bp, sp, nrow = 1, ncol = 2) 上述代码返回两页每页两图multi.page[[1]]

multi.page[[2]]

利用ggarrange()嵌套布局p1 <- ggarrange(sp, bp+font("x.text", size = 9), ncol = 1, nrow = 2) p2 <- ggarrange(density.p, stable.p, text.p, ncol = 1, nrow = 3, heights = c(1, 0.5, 0.3)) ggarrange(p1, p2, ncol = 2, nrow = 1)

SessionInfosessionInfo() ## R version 3.4.1 (2017-06-30) ## Platform: x86_64-w64-mingw32/x64 (64-bit) ## Running under: Windows 10 x64 (build 15063) ## ## Matrix products: default ## ## locale: ## [1] LC_COLLATE=Chinese (Simplified)_China.936 ## [2] LC_CTYPE=Chinese (Simplified)_China.936 ## [3] LC_MONETARY=Chinese (Simplified)_China.936 ## [4] LC_NUMERIC=C ## [5] LC_TIME=Chinese (Simplified)_China.936 ## ## attached base packages: ## [1] grid stats graphics grDevices utils datasets methods ## [8] base ## ## other attached packages: ## [1] survminer_0.4.0 survival_2.41-3 ggpubr_0.1.5 magrittr_1.5 ## [5] cowplot_0.8.0 ggplot2_2.2.1 gridExtra_2.2.1 ## ## loaded via a namespace (and not attached): ## [1] zoo_1.8-0 purrr_0.2.3 reshape2_1.4.2 ## [4] splines_3.4.1 lattice_0.20-35 colorspace_1.3-2 ## [7] htmltools_0.3.6 yaml_2.1.14 survMisc_0.5.4 ## [10] rlang_0.1.2 foreign_0.8-69 glue_1.1.1 ## [13] bindrcpp_0.2 bindr_0.1 plyr_1.8.4 ## [16] stringr_1.2.0 munsell_0.4.3 gtable_0.2.0 ## [19] ggsci_2.7 psych_1.7.5 evaluate_0.10.1 ## [22] labeling_0.3 knitr_1.17 parallel_3.4.1 ## [25] broom_0.4.2 Rcpp_0.12.12 xtable_1.8-2 ## [28] scales_0.4.1 backports_1.1.0 cmprsk_2.2-7 ## [31] km.ci_0.5-2 mnormt_1.5-5 png_0.1-7 ## [34] digest_0.6.12 stringi_1.1.5 dplyr_0.7.2 ## [37] KMsurv_0.1-5 rprojroot_1.2 tools_3.4.1 ## [40] lazyeval_0.2.0 tibble_1.3.3 tidyr_0.7.0 ## [43] pkgconfig_2.0.1 Matrix_1.2-11 data.table_1.10.4 ## [46] assertthat_0.2.0 rmarkdown_1.6 R6_2.2.2 ## [49] nlme_3.1-131 compiler_3.4.1