RFect siRNA转染试剂

产品简介

RFect siRNA转染试剂是一种采用新型的动物源性的纳米材料，毒性很低并且拥有非常卓越转染性能的一种小核酸转染试剂。RFect可用来转染siRNA、antisense RNA、microRNA等200bp以内的小分子RNA和DNA。RFect使用极其简便，先将siRNA与RFect室温混合，再将siRNA-RFect 混合物直接加入含培养基的细胞，血清对转染效果没有影响，不必刻意添加或更换培养液。有关RFect siRNA转染试剂的材料合成和试剂配制我们已申请了国际专利，并通过PCT覆盖国际上多个国家和地区。

产品特点

     · 卓越的细胞转染性能：细胞转染阳性率高达90%以上，基因敲除效果明显，A549细胞        LaminA/C基因敲除效率在95%以上；

· 极低的细胞毒性使实验结果更为客观：转染细胞死亡率不到10%；

· 低浓度siRNA转染获得高基因抑制水平；

· 转染细胞范围非常广，绝大多数贴壁细胞株都能获得比较理想的转染结果（如一般细胞株、肿瘤细胞株等）。

Fig.1. RFect siRNA transfection reagent offers the highest transfection efficiency.  HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using RFect. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A.Labeled siRNA (in red).B.Labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).

Fig..2. RFect siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using RFect  In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig..3.RFect siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using RFect siRNA transfection reagent. RFect concentrations added to the cells  exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR. 

Fig.4.RFect siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using RFect.