以Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening 为例,作者团队开发了一种程序简单,培养时间短的干细胞富集球形模型 (SCESM),适用于抗癌症干细胞化合物的新高通量筛选方法 (HTSs)。
1. J P Hughes. Principles of early drug discovery. Br J Pharmacol. 2011 Mar; 162(6):1239-49. 2. S A Sundberg. High-throughput and ultra-high-throughput screening: solution- and cell-based approaches. Curr Opin Biotechnol. 2000 Feb;11(1):47-53. 3. Vicky M Avery, David Camp, et al. The Identification of Bioactive Natural Products by High Throughput Screening (HTS). Comprehensive Natural Products II. Volume 3, 2010, Pages 177-203. 4. Michael A Dabrowski 1, Kim Dekermendjian, Per-Eric Lund, Johannes J Krupp, Jon Sinclair, Olof Larsson. Ion channel screening technology. CNS Neurol Disord Drug Targets. 2008 Apr;7(2):122-8. 5. Jin-feng Yue, Guan-hua Qiao, Ni Liu, Fa-jun Nan, Zhao-bing Gao. Novel KCNQ2 channel activators discovered using fluorescence-based and automated patch-clamp-based high-throughput screening techniques. Acta Pharmacol Sin. 2016 Jan; 37(1):105-10. 6. Xin Tang, Jesse Drotar, Keji Li, Cullen D Clairmont, Anna Sophie Brumm, Austin J Sullins, Hao Wu, Xiaoxiao Shawn Liu, Jinhua Wang, Nathanael S Gray, Mriganka Sur, Rudolf Jaenisch. Pharmacological enhancement of KCC2 gene expression exerts therapeutic effects on human Rett syndrome neurons and Mecp2 mutant mice. Sci Transl Med. 2019 Jul 31;11(503):eaau0164. 7. Xiao-Tong Hu, Bing-Lin Zhu, Li-Ge Zhao, Jing-Wen Wang, Lu Liu, Yu-Jie Lai, Ling He, Xiao-Juan Deng, Guo-Jun Chen. Histone deacetylase inhibitor apicidin increases expression of the α-secretase ADAM10 through transcription factor USF1-mediated mechanisms. FASEB J. 2017 Apr;31(4):1482-1493. 8. Zhe-Rui Zhang, Hong-Qing Zhang, Xiao-Dan Li, Cheng-Lin Deng, Zhen Wang, Jia-Qi Li, Na Li. Generation and characterization of Japanese encephalitis virus expressing GFP reporter gene for high throughput drug screening. Antiviral Res. 2020 Oct;182:104884. 9. Peng Wang, Juan-Carlos Alvarez-Perez, et al. A high-throughput chemical screen reveals that harmine-mediated inhibition of DYRK1A increases human pancreatic beta cell replication. Nat Med. 2015 Apr;21(4):383-8. 10. Larisa Goričan, Boris Gole, Uroš Potočnik. Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening. Cells. 2020 Jul 16;9(7):1707. 11. Vincent Blay, Bhairavi Tolani, Sunita P Ho, Michelle R Arkin. High-Throughput Screening: today's biochemical and cell-based approaches. Drug Discov Today. 2020 Aug 12; S1359-6446(20)30305-6.