sra转换成fastq

SRA toolkit

fastq-dump

--split-files                    Dump each read into a separate file.Files will received suffix corresponding to read number
--split-3                        Legacy 3-file splitting for mate-pairs:
                                   First 2 biological reads satisfying dumping conditions
                                   are placed in files *_1.fastq and *_2.fastq
                                   If only 1 biological read is dumpable - it is placed in *.fastq

 

http://www.biostars.org/p/11111/